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验证数据展示

  • WB analysis using 68149-1-Ig

    WB analysis using 68149-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68149-1-Ig (WNK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293T using 68149-1-Ig

    FC experiment of HEK-293T using 68149-1-Ig

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human WNK1 (68149-1-Ig, Clone:1B6A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

WNK1 Monoclonal antibody

WNK1 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68149-1-Ig

产品说明书

CloneNo. 1B6A10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

p65, KDP, HSN2, HSAN2, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, A549 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy WNK1 Ready-To-Use IHC Kit. WNK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68149-1-Ig targets WNK1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 WNK1 fusion protein Ag28044 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 WNK lysine deficient protein kinase 1
别名 p65, KDP, HSN2, HSAN2, EC:2.7.11.1
计算分子量 251 kDa
观测分子量250 kDa
GenBank蛋白编号BC021121
基因名称 WNK1
Gene ID (NCBI) 65125
RRIDAB_2923673
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H4A3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.

实验方案

Product Specific Protocols
WB protocol for WNK1 antibody 68149-1-IgDownload protocol
FC protocol for WNK1 antibody 68149-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols