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CoraLite® Plus 488-conjugated WNK1 Monoclonal antibody

WNK1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6A10

Cat no : CL488-68149

Print datasheet

Synonyms

Erythrocyte 65 kDa protein, HSAN2, HSN2, hWNK1, KDP, KIAA0344, Kinase deficient protein, p65, PHA2C, PRKWNK1, PSK, WNK1

验证数据展示

  • IF Staining of A549 using CL488-68149

    IF Staining of A549 using CL488-68149

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 WNK1 antibody (CL488-68149, Clone: 1B6A10 ) at dilution of 1:200.

  • FC experiment of HEK-293T using CL488-68149

    FC experiment of HEK-293T using CL488-68149

    1X10^6 HEK-293T cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human WNK1 (CL488-68149, Clone:1B6A10) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHEK-293T cells
Positive FC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy WNK1 Ready-To-Use IHC Kit. WNK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68149-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68149 targets WNK1 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen WNK1 fusion protein Ag28044 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name WNK lysine deficient protein kinase 1
Synonyms Erythrocyte 65 kDa protein, HSAN2, HSN2, hWNK1, KDP, KIAA0344, Kinase deficient protein, p65, PHA2C, PRKWNK1, PSK, WNK1
Calculated Molecular Weight 251 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC021121
Gene Symbol WNK1
Gene ID (NCBI) 65125
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H4A3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 WNK1 antibody CL488-68149Download protocol
Standard Protocols
Click here to view our Standard Protocols