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验证数据展示

  • IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WNT10B antibody included
  • IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WNT10B antibody included
  • WB analysis using 67210-1-Ig (same clone as 67210-1-PBS)

    WB analysis using 67210-1-Ig (same clone as 67210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67210-1-Ig (WNT10B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67210-1-Ig (same clone as 67210-1-PBS)

    IF Staining of HeLa using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67210-1-Ig (WNT10B antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

WNT10B Monoclonal antibody, PBS Only

WNT10B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67210-1-PBS

产品说明书

CloneNo. 1G11E4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10, WNT10B, WNT12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy WNT10B Ready-To-Use IHC Kit. WNT10B primary antibody included.

产品信息

67210-1-PBS targets WNT10B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 WNT10B fusion protein Ag17114 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 10B
别名 Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10, WNT10B, WNT12
计算分子量 389 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC096353
基因名称 WNT10B
Gene ID (NCBI) 7480
RRIDAB_2882503
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00744
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.