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  • Featured Product
  • KD/KO Validated

WNT10B Monoclonal antibody, PBS Only

WNT10B Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G11E4

Cat No : 67210-1-PBS

Print datasheet

Synonyms

Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10, WNT10B, WNT12

验证数据展示

  • IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WNT10B antibody included
  • IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    IHC staining of mouse liver using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WNT10B antibody included
  • WB analysis using 67210-1-Ig (same clone as 67210-1-PBS)

    WB analysis using 67210-1-Ig (same clone as 67210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67210-1-Ig (WNT10B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67210-1-Ig (same clone as 67210-1-PBS)

    IF Staining of HeLa using 67210-1-Ig (same clone as 67210-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67210-1-Ig (WNT10B antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67210-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy WNT10B Ready-To-Use IHC Kit. WNT10B primary antibody included.

产品信息

67210-1-PBS targets WNT10B in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human
Immunogen WNT10B fusion protein Ag17114 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name wingless-type MMTV integration site family, member 10B
Synonyms Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10, WNT10B, WNT12
Calculated Molecular Weight 389 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC096353
Gene Symbol WNT10B
Gene ID (NCBI) 7480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00744
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.