验证数据展示
经过测试的应用
| Positive WB detected in | NCCIT cells, MCF-7 cells, NCI-H1299 cells, human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | mouse liver tissue, mouse skeletal muscle tissue, human heart tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy WNT10B Ready-To-Use IHC Kit. WNT10B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67210-1-Ig targets WNT10B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human |
| 免疫原 | WNT10B fusion protein Ag17114 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | wingless-type MMTV integration site family, member 10B |
| 别名 | Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10, WNT10B, WNT12 |
| 计算分子量 | 389 aa, 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC096353 |
| 基因名称 | WNT10B |
| Gene ID (NCBI) | 7480 |
| RRID | AB_2882503 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O00744 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WNT10B antibody 67210-1-Ig | Download protocol |
| IHC protocol for WNT10B antibody 67210-1-Ig | Download protocol |
| IF protocol for WNT10B antibody 67210-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Pharmacol Res Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B.
| ||
J Cancer MPP7 mediates EMT via Wnt/β-catenin pathway to promote polarity changes in epithelial ovarian cancer cells | ||
Int J Biol Macromol Xylooligosaccharides alleviate the carbohydrate-enriched diet-induced intestinal barrier dysfunction in carp Megalobrama amblycephala by promoting intestinal development, immunity and gut microbiota | ||
Heliyon MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling |
