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验证数据展示

  • IF Staining of HeLa using CL488-67210

    IF Staining of HeLa using CL488-67210

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 WNT10B antibody (CL488-67210, Clone: 1G11E4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67210

    FC experiment of HeLa using CL488-67210

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human WNT10B (CL488-67210, Clone:1G11E4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated WNT10B Monoclonal antibody

WNT10B Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67210

产品说明书

CloneNo. 1G11E4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC, FC (Intra)

别名

Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10B, WNT12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy WNT10B Ready-To-Use IHC Kit. WNT10B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67210-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67210 targets WNT10B in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human
免疫原 WNT10B fusion protein Ag17114 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 10B
别名 Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10B, WNT12
计算分子量 389 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC096353
基因名称 WNT10B
Gene ID (NCBI) 7480
RRIDAB_2923765
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00744
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 WNT10B antibody CL488-67210Download protocol
FC protocol for CL Plus 488 WNT10B antibody CL488-67210Download protocol
Standard Protocols
Click here to view our Standard Protocols