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WNT2 Polyclonal antibody
WNT2 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue, MCF-7 cells |
Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27214-1-AP targets WNT2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IHC, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | WNT2 fusion protein Ag25845 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | wingless-type MMTV integration site family member 2 |
Synonyms | Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2 |
Calculated Molecular Weight | 40 kDa |
Observed Molecular Weight | 40 kDa, 34 kDa |
GenBank Accession Number | BC029854 |
Gene Symbol | WNT2 |
Gene ID (NCBI) | 7472 |
RRID | AB_2880804 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P09544 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for WNT2 antibody 27214-1-AP | Download protocol |
IHC protocol for WNT2 antibody 27214-1-AP | Download protocol |
IF protocol for WNT2 antibody 27214-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Clin Transl Med Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway. | ||
Aging (Albany NY) LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p. | ||
FASEB J Bisphenol A exposure alters placentation and causes preeclampsia-like features in pregnant mice involved in reprogramming of DNA methylation of WNT2. | ||
Phytother Res Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β-catenin pathway. | ||
Brain Res Bull Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring. | ||
Cancer Med Mycoplasma hyorhinis infection promotes gastric cancer cell motility via β-catenin signaling.
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