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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated WNT2 Polyclonal antibody

WNT2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27214

Print datasheet

Synonyms

IRP, Int-1-related protein, Int-1-like protein 1, INT1L1, Int 1 related protein

验证数据展示

  • IF Staining of MCF-7 using CL488-27214

    IF Staining of MCF-7 using CL488-27214

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 WNT2 antibody (CL488-27214) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-27214

    FC experiment of MCF-7 using CL488-27214

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated WNT2 Polyclonal antibody (CL488-27214)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 27214-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27214 targets WNT2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen WNT2 fusion protein Ag25845 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name wingless-type MMTV integration site family member 2
Synonyms IRP, Int-1-related protein, Int-1-like protein 1, INT1L1, Int 1 related protein
Calculated Molecular Weight 40 kDa
Observed Molecular Weight40 kDa, 34 kDa
GenBank Accession NumberBC029854
Gene Symbol WNT2
Gene ID (NCBI) 7472
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09544
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 WNT2 antibody CL488-27214Download protocol
FC protocol for CL Plus 488 WNT2 antibody CL488-27214Download protocol
Standard Protocols
Click here to view our Standard Protocols