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验证数据展示

  • IF Staining of MCF-7 using CL488-27214

    IF Staining of MCF-7 using CL488-27214

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 WNT2 antibody (CL488-27214) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-27214

    FC experiment of MCF-7 using CL488-27214

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated WNT2 Polyclonal antibody (CL488-27214)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated WNT2 Polyclonal antibody

WNT2 Polyclonal Antibody for IF/ICC
Cat No. CL488-27214

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

IRP, Int-1-related protein, Int-1-like protein 1, INT1L1, Int 1 related protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 27214-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27214 targets WNT2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 WNT2 fusion protein Ag25845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family member 2
别名 IRP, Int-1-related protein, Int-1-like protein 1, INT1L1, Int 1 related protein
计算分子量 40 kDa
观测分子量40 kDa, 34 kDa
GenBank蛋白编号BC029854
基因名称 WNT2
Gene ID (NCBI) 7472
RRIDAB_3672808
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09544
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 WNT2 antibody CL488-27214Download protocol
FC protocol for CL Plus 488 WNT2 antibody CL488-27214Download protocol
Standard Protocols
Click here to view our Standard Protocols