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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 27214-1-AP (same clone as 27214-1-PBS)

    WB analysis using 27214-1-AP (same clone as 27214-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27214-1-PBS in a different storage buffer formulation.

  • WB analysis using 27214-1-AP (same clone as 27214-1-PBS)

    WB analysis using 27214-1-AP (same clone as 27214-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27214-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 27214-1-AP (same clone as 27214-1-PBS)

    IF Staining of MCF-7 using 27214-1-AP (same clone as 27214-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WNT2 antibody (27214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27214-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 27214-1-AP (same clone as 27214-1-PBS)

    IHC staining of rat brain using 27214-1-AP (same clone as 27214-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27214-1-AP (WNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27214-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 27214-1-AP (same clone as 27214-1-PBS)

    FC experiment of Jurkat using 27214-1-AP (same clone as 27214-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human WNT2 (27214-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27214-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

WNT2 Polyclonal antibody, PBS Only

WNT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 27214-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

27214-1-PBS targets WNT2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 WNT2 fusion protein Ag25845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family member 2
别名
计算分子量 40 kDa
观测分子量40 kDa, 34 kDa
GenBank蛋白编号BC029854
基因名称 WNT2
Gene ID (NCBI) 7472
RRIDAB_2880804
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09544
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.