• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

WNT3A Recombinant antibody, PBS Only (Capture/Detector)

WNT3A Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241993G6

Cat no : 84577-3-PBS

Print datasheet

Synonyms

Protein Wnt-3a, Protein Wnt 3a, 241993G6

验证数据展示

  • Sandwich ELISA standard curve of MP01423-3

    Sandwich ELISA standard curve of MP01423-3

    Sandwich ELISA standard curve of MP01423-3, Human WNT3A Recombinant Matched Antibody Pair - PBS only. 84577-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25051. 84577-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01423-3

    Sample test of MP01423-3

    The mean WNT3A concentration was determined to be 999.1 pg/mL in MDA-MB-231 cell extract based on a 1.2 mg/mL extract load and 2,063.4 pg/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

  • Sample test of MP01423-3

    Sample test of MP01423-3

    Serum of six individual healthy human donors was measured. The WNT3A concentration of detected samples was determined to be 2,012.8 pg/mL with a range of 101.5-6,680.8 pg/mL.

  • Cytometric bead array standard curve of MP01423-1

    Cytometric bead array standard curve of MP01423-1

    Cytometric bead array standard curve of MP01423-1, WNT3A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84577-3-PBS. Detection antibody: 84577-1-PBS. Standard: Ag25051. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01423-2

    Cytometric bead array standard curve of MP01423-2

    Cytometric bead array standard curve of MP01423-2, WNT3A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84577-3-PBS. Detection antibody: 84577-2-PBS. Standard: Ag25051. Range: 0.781-100 ng/mL.

产品信息

84577-3-PBS targets WNT3A as part of a matched antibody pair:

MP01423-1: 84577-3-PBS capture and 84577-1-PBS detection (validated in Cytometric bead array)

MP01423-2: 84577-3-PBS capture and 84577-2-PBS detection (validated in Cytometric bead array)

MP01423-3: 84577-5-PBS capture and 84577-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen WNT3A fusion protein Ag25051 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name wingless-type MMTV integration site family, member 3A
Synonyms Protein Wnt-3a, Protein Wnt 3a, 241993G6
Calculated Molecular Weight 352 aa, 39 kDa
GenBank Accession NumberBC103921
Gene Symbol WNT3A
Gene ID (NCBI) 89780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56704
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C