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验证数据展示

  • WB analysis using 30518-1-AP

    WB analysis using 30518-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30518-1-AP (WNT8A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HCT 116 using 30518-1-AP

    IF Staining of HCT 116 using 30518-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using WNT8A antibody (30518-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 30518-1-AP

    IHC staining of human colon cancer using 30518-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30518-1-AP (WNT8A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

WNT8A Polyclonal antibody

WNT8A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30518-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

WNT8D, Protein Wnt-8d, Protein Wnt-8a, Protein Wnt 8d, Protein Wnt 8a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HT-29 cells, SW480 cells, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30518-1-AP targets WNT8A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, rat
Immunogen WNT8A fusion protein Ag29510 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name wingless-type MMTV integration site family, member 8A
Synonyms WNT8D, Protein Wnt-8d, Protein Wnt-8a, Protein Wnt 8d, Protein Wnt 8a
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberNM_001300938
Gene Symbol WNT8A
Gene ID (NCBI) 7478
RRIDAB_3086345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1J5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.

实验方案

Product Specific Protocols
WB protocol for WNT8A antibody 30518-1-APDownload protocol
IHC protocol for WNT8A antibody 30518-1-APDownload protocol
IF protocol for WNT8A antibody 30518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols