WNT8A Polyclonal antibody
WNT8A Polyclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Caco-2 cells, HT-29 cells, SW480 cells, rat heart tissue |
Positive IF detected in | HCT 116 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30518-1-AP targets WNT8A in WB, IF, ELISA applications and shows reactivity with Human, rat samples.
Tested Applications | IF/ICC, WB, ELISA Application Description |
Tested Reactivity | Human, rat |
Immunogen | WNT8A fusion protein Ag29510 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | wingless-type MMTV integration site family, member 8A |
Synonyms | Protein Wnt 8a, Protein Wnt 8d, WNT8A, WNT8D |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 39-45 kDa |
GenBank Accession Number | NM_001300938 |
Gene Symbol | WNT8A |
Gene ID (NCBI) | 7478 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H1J5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
实验方案
Product Specific Protocols | |
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WB protocol for WNT8A antibody 30518-1-AP | Download protocol |
IF protocol for WNT8A antibody 30518-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |