验证数据展示
经过测试的应用
Positive WB detected in | Caco-2 cells, HT-29 cells, SW480 cells, rat heart tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HCT 116 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30518-1-AP targets WNT8A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human, rat |
Immunogen | WNT8A fusion protein Ag29510 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | wingless-type MMTV integration site family, member 8A |
Synonyms | WNT8D, Protein Wnt-8d, Protein Wnt-8a, Protein Wnt 8d, Protein Wnt 8a |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 39-45 kDa |
GenBank Accession Number | NM_001300938 |
Gene Symbol | WNT8A |
Gene ID (NCBI) | 7478 |
RRID | AB_3086345 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H1J5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
实验方案
Product Specific Protocols | |
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WB protocol for WNT8A antibody 30518-1-AP | Download protocol |
IHC protocol for WNT8A antibody 30518-1-AP | Download protocol |
IF protocol for WNT8A antibody 30518-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |