验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HCT 116 cells |
For other applications, we recommend the unconjugated version of this antibody, 30518-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-30518 targets WNT8A in IF/ICC applications and shows reactivity with Human, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, rat |
| 免疫原 | WNT8A fusion protein Ag29510 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | wingless-type MMTV integration site family, member 8A |
| 别名 | Protein Wnt 8a, Protein Wnt 8d, WNT8A, WNT8D |
| 计算分子量 | 39 kDa |
| 观测分子量 | 39-45 kDa |
| GenBank蛋白编号 | NM_001300938 |
| 基因名称 | WNT8A |
| Gene ID (NCBI) | 7478 |
| RRID | AB_3672848 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H1J5 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 WNT8A antibody CL488-30518 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |