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CoraLite® Plus 488-conjugated WNT8A Polyclonal antibody

WNT8A Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-30518

Print datasheet

Synonyms

Protein Wnt 8a, Protein Wnt 8d, WNT8A, WNT8D

验证数据展示

  • IF Staining of HCT 116 using CL488-30518

    IF Staining of HCT 116 using CL488-30518

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using CoraLite® Plus 488 WNT8A antibody (CL488-30518) at dilution of 1:200, CL594-Phalloidin (red).

  • IF Staining of HCT 116 using CL488-30518

    IF Staining of HCT 116 using CL488-30518

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using CoraLite® Plus 488 WNT8A antibody (CL488-30518) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHCT 116 cells
For other applications, we recommend the unconjugated version of this antibody, 30518-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-30518 targets WNT8A in IF/ICC applications and shows reactivity with Human, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, rat
Immunogen WNT8A fusion protein Ag29510 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name wingless-type MMTV integration site family, member 8A
Synonyms Protein Wnt 8a, Protein Wnt 8d, WNT8A, WNT8D
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberNM_001300938
Gene Symbol WNT8A
Gene ID (NCBI) 7478
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1J5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 WNT8A antibody CL488-30518Download protocol
Standard Protocols
Click here to view our Standard Protocols