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  • Featured Product
  • KD/KO Validated

WTAP Polyclonal antibody, PBS Only

WTAP Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10200-1-PBS

Print datasheet

Synonyms

Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, Mum2, KIAA0105, FL2D

验证数据展示

  • WB analysis of HeLa using 10200-1-AP (same clone as 10200-1-PBS)

    WB analysis of HeLa using 10200-1-AP (same clone as 10200-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat cells using 10200-1-AP (same clone as 10200-1-PBS)

    WB analysis of Jurkat cells using 10200-1-AP (same clone as 10200-1-PBS)

    WB result of WTAP specific antibody (10200-1-AP, 1:6000) with si-control and si-WTAP transfected Jurkat cells. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10200-1-AP (same clone as 10200-1-PBS)

    IP experiment of HEK-293 using 10200-1-AP (same clone as 10200-1-PBS)

    IP result of anti-WTAP (IP:10200-1-AP, 3ug; Detection:10200-1-AP 1:500) with HEK-293 cells lysate 2500ug. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 10200-1-AP (same clone as 10200-1-PBS)

    IP experiment of Jurkat using 10200-1-AP (same clone as 10200-1-PBS)

    IP result of anti-WTAP (IP:10200-1-AP, 4ug; Detection:10200-1-AP 1:8000) with Jurkat cells lysate 2280 ug. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 10200-1-AP (same clone as 10200-1-PBS)

    IHC staining of human stomach using 10200-1-AP (same clone as 10200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10200-1-AP (WTAP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WTAP antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 10200-1-AP (same clone as 10200-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 10200-1-AP (same clone as 10200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10200-1-AP (WTAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WTAP antibody included
Planning an IHC experiment? We recommend our IHCeasy WTAP Ready-To-Use IHC Kit. WTAP primary antibody included.

产品信息

10200-1-PBS targets WTAP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen WTAP fusion protein Ag0259 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Wilms tumor 1 associated protein
Synonyms Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, Mum2, KIAA0105, FL2D
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000383
Gene Symbol WTAP
Gene ID (NCBI) 9589
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15007
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 50-55 kDa due to phosphorylation.