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验证数据展示

  • WB analysis of HepG2 using 28689-1-AP

    WB analysis of HepG2 using 28689-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28689-1-AP

    IHC staining of human breast cancer using 28689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WWP1 antibody included
  • IHC staining of human breast cancer using 28689-1-AP

    IHC staining of human breast cancer using 28689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WWP1 antibody included

WWP1 Polyclonal antibody

WWP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28689-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Synonyms

AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, A431 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy WWP1 Ready-To-Use IHC Kit. WWP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28689-1-AP targets WWP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen WWP1 fusion protein Ag30161 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WW domain containing E3 ubiquitin protein ligase 1
Synonyms AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1
Calculated Molecular Weight 922 aa, 105 kDa
Observed Molecular Weight 95-105 kDa
GenBank Accession NumberBC036065
Gene Symbol WWP1
Gene ID (NCBI) 11059
RRIDAB_2881195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0M0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for WWP1 antibody 28689-1-APDownload protocol
IHC protocol for WWP1 antibody 28689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Med

Quercetin-induced degradation of RhoC suppresses hepatocellular carcinoma invasion and metastasis

Authors - Chunlong Huang
View Article
humanWB,IHC

Cell Mol Biol Lett

Extrachromosomal circular DNA promotes prostate cancer progression through the FAM84B/CDKN1B/MYC/WWP1 axis

Authors - Wei Jin
View Article