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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of PC-3 using CL488-67804

    FC experiment of PC-3 using CL488-67804

    1x10^6 PC-3 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Wwp1 Monoclonal Antibody (CL488-67804, Clone:2G8G11)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated WWP1 Monoclonal antibody

WWP1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67804

产品说明书

CloneNo. 2G8G11

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

FC (Intra)

别名

hSDRP1, HECT-type E3 ubiquitin transferase WWP1, EC:2.3.2.26, Atrophin-1-interacting protein 5, AIP5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy WWP1 Ready-To-Use IHC Kit. WWP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67804-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67804 targets WWP1 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 WWP1 fusion protein Ag30171 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 WW domain containing E3 ubiquitin protein ligase 1
别名 hSDRP1, HECT-type E3 ubiquitin transferase WWP1, EC:2.3.2.26, Atrophin-1-interacting protein 5, AIP5
计算分子量 922 aa, 105 kDa
观测分子量 95-105 kDa
GenBank蛋白编号BC036065
基因名称 WWP1
Gene ID (NCBI) 11059
RRIDAB_3672963
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0M0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 WWP1 antibody CL488-67804Download protocol
Standard Protocols
Click here to view our Standard Protocols