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验证数据展示

  • FC experiment of PC-3 using CL488-67804

    FC experiment of PC-3 using CL488-67804

    1x10^6 PC-3 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Wwp1 Monoclonal Antibody (CL488-67804, Clone:2G8G11)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated WWP1 Monoclonal antibody

WWP1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67804

Print datasheet

CloneNo. 2G8G11

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Synonyms

hSDRP1, HECT-type E3 ubiquitin transferase WWP1, EC:2.3.2.26, Atrophin-1-interacting protein 5, AIP5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy WWP1 Ready-To-Use IHC Kit. WWP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67804-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67804 targets WWP1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen WWP1 fusion protein Ag30171 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name WW domain containing E3 ubiquitin protein ligase 1
Synonyms hSDRP1, HECT-type E3 ubiquitin transferase WWP1, EC:2.3.2.26, Atrophin-1-interacting protein 5, AIP5
Calculated Molecular Weight 922 aa, 105 kDa
Observed Molecular Weight 95-105 kDa
GenBank Accession NumberBC036065
Gene Symbol WWP1
Gene ID (NCBI) 11059
RRIDAB_3672963
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0M0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 WWP1 antibody CL488-67804Download protocol
Standard Protocols
Click here to view our Standard Protocols