验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, LNCap cells, MCF-7 cells, K-562 cells, HSC-T6 cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
67274-1-Ig targets WWP2 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, Rat |
Cited Reactivity | human |
Immunogen | WWP2 fusion protein Ag2835 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | WW domain containing E3 ubiquitin protein ligase 2 |
Synonyms | AIP2, WW domain containing protein 2, WWP2, WWp2 like |
Calculated Molecular Weight | 870 aa, 99 kDa |
Observed Molecular Weight | 100-115 kDa |
GenBank Accession Number | BC013645 |
Gene Symbol | WWP2 |
Gene ID (NCBI) | 11060 |
RRID | AB_2882543 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O00308 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WWP2 (WW domain-containing protein 2) is also named as AIP2 and belongs to the NEDD4-like protein family. It functions as an E3 ubiquitin ligase for PTEN that plays a vital role in tumour-cell survival and is also involved in regulating transcription, embryonic stem-cell fate, cellular transport and T-cell activation processes (PMID:21532586). WWP2, which specifically interacted with ES cell-specific transcription factor Oct-4 and promoted its ubiquitination both in vitro and in vivo, is a ubiquitously expressed protein and exists in both cytoplasmic and nuclear compartments in ES cells (PMID:15047715).
实验方案
Product Specific Protocols | |
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WB protocol for WWP2 antibody 67274-1-Ig | Download protocol |
IHC protocol for WWP2 antibody 67274-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |