验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, LN229 cells, MDA-MB-453s cells |
| Positive IP detected in | MDA-MB-453s cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat kidney tissue |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23306-1-AP targets WWTR1/TAZ in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | WWTR1/TAZ fusion protein Ag19659 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WW domain containing transcription regulator 1 |
| 别名 | TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif |
| 计算分子量 | 44 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC014052 |
| 基因名称 | WWTR1 |
| Gene ID (NCBI) | 25937 |
| RRID | AB_2721185 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZV5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma (PMID: 22003437, PMID: 27764783). TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WWTR1/TAZ antibody 23306-1-AP | Download protocol |
| IHC protocol for WWTR1/TAZ antibody 23306-1-AP | Download protocol |
| IF protocol for WWTR1/TAZ antibody 23306-1-AP | Download protocol |
| IP protocol for WWTR1/TAZ antibody 23306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Programming of Schwann Cells by Lats1/2-TAZ/YAP Signaling Drives Malignant Peripheral Nerve Sheath Tumorigenesis. | ||
Nat Neurosci YAP and TAZ control peripheral myelination and the expression of laminin receptors in Schwann cells.
| ||
Sci Adv YAP drives fate conversion and chemoresistance of small cell lung cancer.
| ||
Bone Res TAZ inhibits osteoclastogenesis by attenuating TAK1/NF-κB signaling.
| ||
Clin Transl Med NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA stabilization |
