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验证数据展示

  • IF Staining of U-251 using CL488-23306

    IF Staining of U-251 using CL488-23306

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 WWTR1 antibody (CL488-23306) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-23306

    FC experiment of HeLa using CL488-23306

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human WWTR1 (CL488-23306)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated WWTR1/TAZ Polyclonal antibody

WWTR1/TAZ Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-23306

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IF/ICC, FC (Intra)

别名

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 23306-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-23306 targets WWTR1/TAZ in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat
免疫原 WWTR1/TAZ fusion protein Ag19659 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WW domain containing transcription regulator 1
别名 TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif
计算分子量 44 kDa
观测分子量 50 kDa
GenBank蛋白编号BC014052
基因名称 WWTR1
Gene ID (NCBI) 25937
RRIDAB_3672754
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZV5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 WWTR1/TAZ antibody CL488-23306Download protocol
FC protocol for CL Plus 488 WWTR1/TAZ antibody CL488-23306Download protocol
Standard Protocols
Click here to view our Standard Protocols