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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83959-5-RR (same clone as 83959-5-PBS)

    WB analysis using 83959-5-RR (same clone as 83959-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83959-5-RR (XBP1S-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 83959-5-RR (same clone as 83959-5-PBS)

    IHC staining of mouse liver using 83959-5-RR (same clone as 83959-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-5-RR (XBP1S-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

  • FC experiment of Ramos using 83959-5-RR (same clone as 83959-5-PBS)

    FC experiment of Ramos using 83959-5-RR (same clone as 83959-5-PBS)

    1x10^6 Ramos cells were intracellularly stained with 0.25 ug XBP1S-specific Recombinant antibody (83959-5-RR, Clone:241004B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83959-5-RR

    Affinity and Kinetic Characterization of 83959-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83959-5-RR against Human XBP1S-specific were performed. The affinity constant is 1.08 nM.

XBP1S-specific Recombinant antibody, PBS Only

XBP1S-specific Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83959-5-PBS

产品说明书

CloneNo. 241004B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

XBP1, 241004B6, Tax-responsive element-binding protein 5, TREB-5, X box binding protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83959-5-PBS targets XBP1S-specific in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 XBP1S-specific fusion protein Ag21703 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 X-box binding protein 1
别名 XBP1, 241004B6, Tax-responsive element-binding protein 5, TREB-5, X box binding protein 1
计算分子量 261 aa, 29 kDa
观测分子量60 kDa
GenBank蛋白编号BC000938
基因名称 XBP1
Gene ID (NCBI) 7494
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17861
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The calculated molecular weight of protein generated from the unspliced XBP1 isoform XBP1U mRNA is 33 kDa, and protein generated from spliced XBP1 isoform XBP1S mRNA is 54 kDa. This antibody specially recognizes the XBP1S isoform.