XBP1S-specific Recombinant antibody
XBP1S-specific Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
241004B6
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HuH-7 cells, Raji cells, HEK-293T cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | Ramos cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83959-5-RR targets XBP1S-specific in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | XBP1S-specific fusion protein Ag21703 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | X-box binding protein 1 |
Synonyms | XBP1, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1, TREB-5 |
Calculated Molecular Weight | 261 aa, 29 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC000938 |
Gene Symbol | XBP1 |
Gene ID (NCBI) | 7494 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P17861 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for XBP1S-specific antibody 83959-5-RR | Download protocol |
IHC protocol for XBP1S-specific antibody 83959-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |