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XBP1S Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 39.1-2500 pg/mL

Cat No : MP00917-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00917-4, Human XBP1S Recombinant Matched Antibody Pair - PBS only. 83959-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag21703. 83959-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

    Sandwich ELISA standard curve of MP00917-4

    Sandwich ELISA standard curve of MP00917-4, Human XBP1S Recombinant Matched Antibody Pair - PBS only. 83959-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag21703. 83959-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • The mean XBP1S concentration was determined to be 451.7 pg/mL in T-47D cell extract based on a 4.5 mg/mL extract load and 423.7 pg/mL in HEK-293T cell extract based on a 1.4 mg/mL extract load.

    Sample test of MP00917-4

    The mean XBP1S concentration was determined to be 451.7 pg/mL in T-47D cell extract based on a 4.5 mg/mL extract load and 423.7 pg/mL in HEK-293T cell extract based on a 1.4 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00917-4 targets XBP1S in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: XBP1S-specific Recombinant antibody, PBS Only (Capture) 83959-7-PBS (241004A5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: XBP1S-specific Recombinant antibody, PBS Only (Detector) 83959-1-PBS (241004E1). 100 μg. Concentration 1 mgl/ml.

Alternative XBP1S matched antibody pairs: MP00917-1, MP00917-2, MP00917-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83959-7-PBS
Tested Reactivity human
Immunogen XBP1S-specific fusion protein Ag21703
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name X-box binding protein 1
Synonyms XBP1, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1, TREB-5
GenBank accession numberBC000938
Gene symbol XBP1
UniprotP17861
Gene ID (NCBI) 7494
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83959-1-PBS
Tested Reactivity human
Immunogen XBP1S-specific fusion protein Ag21703
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name X-box binding protein 1
Synonyms XBP1, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1, TREB-5
GenBank accession numberBC000938
Gene symbol XBP1
UniprotP17861
Gene ID (NCBI) 7494
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C