验证数据展示
经过测试的应用
| Positive IF-P detected in | human colon cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy XIAP Ready-To-Use IHC Kit. XIAP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66800-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66800 targets XIAP in IF-P applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | XIAP fusion protein Ag18088 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | X-linked inhibitor of apoptosis |
| 别名 | API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2 |
| 计算分子量 | 60 kDa |
| GenBank蛋白编号 | BC032729 |
| 基因名称 | XIAP |
| Gene ID (NCBI) | 331 |
| RRID | AB_2919373 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P98170 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 XIAP antibody CL488-66800 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |