• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

XLF Monoclonal antibody, PBS Only

XLF Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F8C7

Cat no : 66552-1-PBS

Print datasheet

Synonyms

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

验证数据展示

  • WB analysis using 66552-1-Ig (same clone as 66552-1-PBS)

    WB analysis using 66552-1-Ig (same clone as 66552-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66552-1-Ig (XLF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

产品信息

66552-1-PBS targets XLF in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen XLF fusion protein Ag2496 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nonhomologous end-joining factor 1
Synonyms NHEJ1, Protein cernunnos, XLF, XRCC4 like factor
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC030986
Gene Symbol XLF
Gene ID (NCBI) 79840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H9Q4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.