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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66552-1-Ig (same clone as 66552-1-PBS)

    WB analysis using 66552-1-Ig (same clone as 66552-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66552-1-Ig (XLF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

XLF Monoclonal antibody, PBS Only

XLF Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66552-1-PBS

产品说明书

CloneNo. 1F8C7

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, Indirect ELISA

别名

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66552-1-PBS targets XLF in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 XLF fusion protein Ag2496 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nonhomologous end-joining factor 1
别名 NHEJ1, Protein cernunnos, XLF, XRCC4 like factor
计算分子量 35 kDa
观测分子量 37 kDa
GenBank蛋白编号BC030986
基因名称 XLF
Gene ID (NCBI) 79840
RRIDAB_2881914
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H9Q4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.