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验证数据展示

  • WB analysis using 66552-1-Ig

    WB analysis using 66552-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66552-1-Ig (XLF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 66552-1-Ig

    IHC staining of human testis using 66552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 66552-1-Ig

    IHC staining of human testis using 66552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

XLF Monoclonal antibody

XLF Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66552-1-Ig

产品说明书

CloneNo. 1F8C7

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, ELISA

别名

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-30000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66552-1-Ig targets XLF in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 XLF fusion protein Ag2496 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nonhomologous end-joining factor 1
别名 NHEJ1, Protein cernunnos, XLF, XRCC4 like factor
计算分子量 35 kDa
观测分子量 37 kDa
GenBank蛋白编号BC030986
基因名称 XLF
Gene ID (NCBI) 79840
RRIDAB_2881914
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H9Q4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.

实验方案

Product Specific Protocols
WB protocol for XLF antibody 66552-1-IgDownload protocol
IHC protocol for XLF antibody 66552-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols