验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 66552-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-66552 targets XLF in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human |
| 免疫原 | XLF fusion protein Ag2496 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | nonhomologous end-joining factor 1 |
| 别名 | FLJ12610, NHEJ1, Protein cernunnos, XLF, XRCC4 like factor |
| 计算分子量 | 35 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC030986 |
| 基因名称 | XLF |
| Gene ID (NCBI) | 79840 |
| RRID | AB_3084894 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H9Q4 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 XLF antibody CL750-66552 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |