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CoraLite® Plus 750-conjugated XLF Monoclonal antibody

XLF Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1F8C7

Cat no : CL750-66552

Print datasheet

Synonyms

FLJ12610, NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

验证数据展示

  • WB analysis using CL750-66552

    WB analysis using CL750-66552

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66552 (XLF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66552-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66552 targets XLF in WB applications and shows reactivity with Human samples.

Tested Applications WB Application Description
Tested Reactivity Human
Immunogen XLF fusion protein Ag2496 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nonhomologous end-joining factor 1
Synonyms FLJ12610, NHEJ1, Protein cernunnos, XLF, XRCC4 like factor
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC030986
Gene Symbol XLF
Gene ID (NCBI) 79840
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H9Q4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 XLF antibody CL750-66552Download protocol
Standard Protocols
Click here to view our Standard Protocols