验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | L02 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IF | See 2 publications below |
产品信息
16462-1-AP targets XPA in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, zebrafish |
| Immunogen | XPA fusion protein Ag9536 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | xeroderma pigmentosum, complementation group A |
| Synonyms | XPAC, XP1, Xeroderma pigmentosum group A-complementing protein, DNA repair protein complementing XP-A cells |
| Calculated Molecular Weight | 374aa,42 kDa; 273aa,31 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC014965 |
| Gene Symbol | XPA |
| Gene ID (NCBI) | 7507 |
| RRID | AB_2878261 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23025 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XPA antibody 16462-1-AP | Download protocol |
| IF protocol for XPA antibody 16462-1-AP | Download protocol |
| IP protocol for XPA antibody 16462-1-AP | Download protocol |
| FC protocol for XPA antibody 16462-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Macromolecular NO-Donor Micelles for Targeted and Augmented Chemotherapy against Prostate Cancer | ||
Front Pharmacol Triptolide enhances carboplatin-induced apoptosis by inhibiting nucleotide excision repair (NER) activity in melanoma | ||
Sci Total Environ AHR-mediated DNA damage contributes to BaP-induced cardiac malformations in zebrafish |
