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验证数据展示

  • FC experiment of HepG2 using CL488-16462

    FC experiment of HepG2 using CL488-16462

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human XPA (CL488-16462) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated XPA Polyclonal antibody

XPA Polyclonal Antibody for FC (Intra)
Cat No. CL488-16462

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

XPAC, XP1, Xeroderma pigmentosum group A-complementing protein, DNA repair protein complementing XP-A cells

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy XPA Ready-To-Use IHC Kit. XPA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16462-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16462 targets XPA in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 XPA fusion protein Ag9536 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 xeroderma pigmentosum, complementation group A
别名 XPAC, XP1, Xeroderma pigmentosum group A-complementing protein, DNA repair protein complementing XP-A cells
计算分子量374aa,42 kDa; 273aa,31 kDa
观测分子量 40 kDa
GenBank蛋白编号BC014965
基因名称 XPA
Gene ID (NCBI) 7507
RRIDAB_3083987
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23025
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 XPA antibody CL488-16462Download protocol
Standard Protocols
Click here to view our Standard Protocols