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验证数据展示

  • WB analysis of HEK-293 using 66652-1-Ig (same clone as 66652-1-PBS)

    WB analysis of HEK-293 using 66652-1-Ig (same clone as 66652-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

    IF Staining of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66652-1-Ig (XRCC2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

    IF Staining of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC2 antibody (66652-1-Ig, Clone: 3D9A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

    FC experiment of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRCC2 (66652-1-Ig, Clone:3D9A1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

XRCC2 Monoclonal antibody, PBS Only

XRCC2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66652-1-PBS

产品说明书

CloneNo. 3D9A1

宿主/亚型

Mouse / IgG2b

种属反应性

mouse, human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

X-ray repair cross-complementing protein 2, DNA repair protein XRCC2, 3D9A1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66652-1-PBS targets XRCC2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse, human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 mouse, human
免疫原 XRCC2 fusion protein Ag14421 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 X-ray repair complementing defective repair in Chinese hamster cells 2
别名 X-ray repair cross-complementing protein 2, DNA repair protein XRCC2, 3D9A1
计算分子量 280 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC042137
基因名称 XRCC2
Gene ID (NCBI) 7516
RRIDAB_2882009
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43543
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.