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CoraLite®594-conjugated XRCC2 Monoclonal antibody

XRCC2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D9A1

Cat no : CL594-66652

Print datasheet

Synonyms

X-ray repair cross-complementing protein 2, DNA repair protein XRCC2, 3D9A1

验证数据展示

  • IF Staining of L02 using CL594-66652

    IF Staining of L02 using CL594-66652

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite®594-conjugated XRCC2 antibody (CL594-66652, Clone: 3D9A1 ) at dilution of 1:100, CL488-Phalloidin (green).

  • FC experiment of HepG2 using CL594-66652

    FC experiment of HepG2 using CL594-66652

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human XRCC2 (CL594-66652, Clone:3D9A1) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inL02 cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66652-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66652 targets XRCC2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen XRCC2 fusion protein Ag14421 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 2
Synonyms X-ray repair cross-complementing protein 2, DNA repair protein XRCC2, 3D9A1
Calculated Molecular Weight 280 aa, 32 kDa
GenBank Accession NumberBC042137
Gene Symbol XRCC2
Gene ID (NCBI) 7516
RRIDAB_2883610
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43543
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.

实验方案

Product Specific Protocols
IF protocol for CL594 XRCC2 antibody CL594-66652Download protocol
FC protocol for CL594 XRCC2 antibody CL594-66652Download protocol
Standard Protocols
Click here to view our Standard Protocols