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验证数据展示

  • FC experiment of HepG2 using CL488-66852

    FC experiment of HepG2 using CL488-66852

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human XRN2 (CL488-66852, Clone:2C3E3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated XRN2 Monoclonal antibody

XRN2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66852

产品说明书

CloneNo. 2C3E3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

DHM1-like protein, DHM1 like protein, 5'-3' exoribonuclease 2, 5' 3' exoribonuclease 2, 5 3 exoribonuclease 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy XRN2 Ready-To-Use IHC Kit. XRN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66852-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66852 targets XRN2 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 XRN2 fusion protein Ag27927 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 5'-3' exoribonuclease 2
别名 DHM1-like protein, DHM1 like protein, 5'-3' exoribonuclease 2, 5' 3' exoribonuclease 2, 5 3 exoribonuclease 2
计算分子量 104 kDa
观测分子量 109 kDa
GenBank蛋白编号BC006417
基因名称 XRN2
Gene ID (NCBI) 22803
RRIDAB_3084279
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H0D6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 XRN2 antibody CL488-66852Download protocol
Standard Protocols
Click here to view our Standard Protocols