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验证数据展示

  • FC experiment of A431 using CL488-67911

    FC experiment of A431 using CL488-67911

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human YTHDC1 (CL488-67911, Clone:1F5G11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated YTHDC1 Monoclonal antibody

YTHDC1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67911

产品说明书

CloneNo. 1F5G11

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

YTHDC1, 1F5G11, KIAA1966, Splicing factor YT521, YT521, YT521 B, YT521-B, YTH domain-containing protein 1, YTHDC 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDC1 Ready-To-Use IHC Kit. YTHDC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67911-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67911 targets YTHDC1 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 YTHDC1 fusion protein Ag6289 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 YTH domain containing 1
别名 YTHDC1, 1F5G11, KIAA1966, Splicing factor YT521, YT521, YT521 B, YT521-B, YTH domain-containing protein 1, YTHDC 1
计算分子量 85 kDa
观测分子量~100 kDa
GenBank蛋白编号BC053863
基因名称 YTHDC1
Gene ID (NCBI) 91746
RRIDAB_2934588
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96MU7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 YTHDC1 antibody CL488-67911Download protocol
Standard Protocols
Click here to view our Standard Protocols