验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, HCT 116 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-12 cells |
Planning an IHC experiment? We recommend our IHCeasy YTHDF3 Ready-To-Use IHC Kit. YTHDF3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25537-1-AP targets YTHDF3-specific in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig, chicken |
| 免疫原 | YTHDF3-specific fusion protein Ag22374 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | YTH domain family, member 3 |
| 别名 | YTH domain family protein 3, YTH domain family, member 3, YTHDF3, YTHDF3-specific |
| 计算分子量 | 585 aa, 64 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC052970 |
| 基因名称 | YTHDF3 |
| Gene ID (NCBI) | 253943 |
| RRID | AB_2847817 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z739 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDF3-specific antibody 25537-1-AP | Download protocol |
| IHC protocol for YTHDF3-specific antibody 25537-1-AP | Download protocol |
| IF protocol for YTHDF3-specific antibody 25537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis
| ||
Cell Mol Immunol Inhibition of METTL3 in macrophages provides protection against intestinal inflammation | ||
Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins | ||
Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. | ||
Nat Commun Circular RNA encoded MET variant promotes glioblastoma tumorigenesis
|