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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated YTHDF3-specific Polyclonal antibody

YTHDF3-specific Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25537

Print datasheet

Synonyms

FLJ31657, YTH domain family protein 3, YTH domain family, member 3, YTHDF3

验证数据展示

  • IF Staining of PC-12 using CL488-25537

    IF Staining of PC-12 using CL488-25537

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 YTHDF3-specific antibody (CL488-25537) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF3 Ready-To-Use IHC Kit. YTHDF3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25537-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25537 targets YTHDF3-specific in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen YTHDF3-specific fusion protein Ag22374 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name YTH domain family, member 3
Synonyms FLJ31657, YTH domain family protein 3, YTH domain family, member 3, YTHDF3
Calculated Molecular Weight 585 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC052970
Gene Symbol YTHDF3
Gene ID (NCBI) 253943
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z739
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 YTHDF3-specific antibody CL488-25537Download protocol
Standard Protocols
Click here to view our Standard Protocols