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CoraLite® Plus 488-conjugated YWHAZ Monoclonal antibody

YWHAZ Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G12B10

Cat no : CL488-67698

Print datasheet

Synonyms

KCIP-1, KCIP 1, 3G12B10, 14-3-3 protein zeta/delta, 14-3-3

验证数据展示

  • IF Staining of MCF-7 using CL488-67698

    IF Staining of MCF-7 using CL488-67698

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 YWHAZ antibody (CL488-67698, Clone: 3G12B10 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-67698

    FC experiment of MCF-7 using CL488-67698

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated YWHAZ Monoclonal antibody (CL488-67698, Clone:3G12B10)(red), or 0.4 ug Isotype Control (CL488-66360-1) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy YWHAZ Ready-To-Use IHC Kit. YWHAZ primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67698-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67698 targets YWHAZ in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen YWHAZ fusion protein Ag6667 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Synonyms KCIP-1, KCIP 1, 3G12B10, 14-3-3 protein zeta/delta, 14-3-3
Calculated Molecular Weight 28 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC003623
Gene Symbol YWHAZ
Gene ID (NCBI) 7534
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP63104
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 YWHAZ antibody CL488-67698Download protocol
FC protocol for CL Plus 488 YWHAZ antibody CL488-67698Download protocol
Standard Protocols
Click here to view our Standard Protocols