验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55270-1-AP targets ZBTB33 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | IHC, IF, CoIP, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger and BTB domain containing 33 |
| 别名 | ZNF348, ZNF kaiso, Zinc finger and BTB domain-containing protein 33, Transcriptional regulator Kaiso, KAISO |
| 计算分子量 | 74 kDa |
| 观测分子量 | 68-74 kDa |
| GenBank蛋白编号 | NM_006777 |
| 基因名称 | ZBTB33 |
| Gene ID (NCBI) | 10009 |
| RRID | AB_2881295 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86T24 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZBTB33, also named as Transcriptional regulator Kaiso, is a 672 amino acid protein, which localizes in nucleus and cytoplasm. ZBTB33 as a transcriptional regulator with bimodal DNA-binding specificity binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters and may contribute to the repression of target genes of the Wnt signaling pathway. The calculated molecular weight of ZBTB33 is 75 kDa, but modified protein is about 100 kDa (PMID: 10207085 and PMID: 16354691)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZBTB33 antibody 55270-1-AP | Download protocol |
| IHC protocol for ZBTB33 antibody 55270-1-AP | Download protocol |
| IP protocol for ZBTB33 antibody 55270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun ZNF131 facilitates the growth of hepatocellular carcinoma by acting as a transcriptional activator of SMC4 expression |