• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of A431 using 55270-1-AP

    WB analysis of A431 using 55270-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 55270-1-AP (ZBTB33 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 55270-1-AP

    IP experiment of A431 using 55270-1-AP

    IP result of anti-ZBTB33 (IP:55270-1-AP, 4ug; Detection:55270-1-AP 1:300) with A431 cells lysate 1200ug.

  • IHC staining of human ovary cancer using 55270-1-AP

    IHC staining of human ovary cancer using 55270-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 55270-1-AP (ZBTB33 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZBTB33 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 55270-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 55270-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 55270-1-AP (ZBTB33 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZBTB33 antibody included

ZBTB33 Polyclonal antibody

ZBTB33 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 55270-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA and More (3)

Synonyms

ZNF348, ZNF kaiso, Zinc finger and BTB domain-containing protein 33, Transcriptional regulator Kaiso, KAISO

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ZBTB33 Ready-To-Use IHC Kit. ZBTB33 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55270-1-AP targets ZBTB33 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsIHC, IF, CoIP, ChIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger and BTB domain containing 33
Synonyms ZNF348, ZNF kaiso, Zinc finger and BTB domain-containing protein 33, Transcriptional regulator Kaiso, KAISO
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 68-74 kDa
GenBank Accession NumberNM_006777
Gene Symbol ZBTB33
Gene ID (NCBI) 10009
RRIDAB_2881295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86T24
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB33, also named as Transcriptional regulator Kaiso, is a 672 amino acid protein, which localizes in nucleus and cytoplasm. ZBTB33 as a transcriptional regulator with bimodal DNA-binding specificity binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters and may contribute to the repression of target genes of the Wnt signaling pathway. The calculated molecular weight of ZBTB33 is 75 kDa, but modified protein is about 100 kDa (PMID: 10207085 and PMID: 16354691)

实验方案

Product Specific Protocols
WB protocol for ZBTB33 antibody 55270-1-APDownload protocol
IHC protocol for ZBTB33 antibody 55270-1-APDownload protocol
IP protocol for ZBTB33 antibody 55270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Front Immunol

Diagnostic value of anti-Kaiso autoantibody in axial spondyloarthritis

Authors - Xinzhe Feng
View Article
humanCoIP,ChIP

Biochem Biophys Res Commun

ZNF131 facilitates the growth of hepatocellular carcinoma by acting as a transcriptional activator of SMC4 expression

Authors - Meng Jin
View Article