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IHCeasy ZBTB33 Ready-To-Use IHC Kit

ZBTB33 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC1805

Synonyms

KAISO, ZBTB33, ZNF kaiso, ZNF348

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC1805 (ZBTB33 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC1805 (ZBTB33 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1805 (ZBTB33 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1805 (ZBTB33 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using KHC1805 (ZBTB33 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using KHC1805 (ZBTB33 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using KHC1805 (ZBTB33 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using KHC1805 (ZBTB33 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using KHC1805 (ZBTB33 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using KHC1805 (ZBTB33 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using KHC1805 (ZBTB33 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using KHC1805 (ZBTB33 IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC1805 is a ready-to-use IHC kit for staining of ZBTB33. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy ZBTB33 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

ZBTB33, also named as Transcriptional regulator Kaiso, is a 672 amino acid protein, which localizes in nucleus and cytoplasm. ZBTB33 as a transcriptional regulator with bimodal DNA-binding specificity binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters and may contribute to the repression of target genes of the Wnt signaling pathway.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms KAISO, ZBTB33, ZNF kaiso, ZNF348