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验证数据展示

  • IF Staining of HepG2 using CL488-83616-5

    IF Staining of HepG2 using CL488-83616-5

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ZBTB7A antibody (CL488-83616-5, Clone: 240555A6 ) at dilution of 1:200, CL594-Phalloidin (red).

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  • KD/KO验证

CoraLite® Plus 488-conjugated ZBTB7A Recombinant antibody

ZBTB7A Recombinant Antibody for IF/ICC
Cat No. CL488-83616-5

产品说明书

CloneNo. 240555A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83616-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83616-5 targets ZBTB7A in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger and BTB domain containing 7A
别名
计算分子量 61 kDa
观测分子量61 kDa, 75-80 kDa
GenBank蛋白编号BC113511
基因名称 ZBTB7A
Gene ID (NCBI) 51341
RRIDAB_3673283
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95365
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB7A, also named as LRF, FBI1, FBI-1, pokemon, ZBTB7, ZNF857A and DKFZp547O146, a member of the POK (POZ and Krüppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis (PMID: 35798238). ZBTB7A is involved in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. ZBTB7A is widely expressed in normal thymus and it is aberrantly overexpressed in human cancers (PMID: 28942243). ZBTB7A has 10 potential sumoylation sites located at lysine 61, 354, 371, 379, 383, 396, 486, 487, 536 and 539 (PMID: 20471975, 19471103). ZBTB7A has a predicted molecular weight of 61 kDa and the 75~80-kDa band could be the sumoylated form.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ZBTB7A antibody CL488-83616-5Download protocol
Standard Protocols
Click here to view our Standard Protocols