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ZC3H8 Monoclonal antibody

ZC3H8 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G8C6

Cat no : 68282-1-Ig

Print datasheet

Synonyms

Zinc finger CCCH domain-containing protein 8, ZC3HDC8, Fliz1, 1G8C6

验证数据展示

  • WB analysis using 68282-1-Ig

    WB analysis using 68282-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68282-1-Ig (ZC3H8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of HEK-293 using 68282-1-Ig

    FC experiment of HEK-293 using 68282-1-Ig

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ZC3H8 Monoclonal antibody (68282-1-Ig, Clone:1G8C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68282-1-Ig targets ZC3H8 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ZC3H8 fusion protein Ag23496 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zinc finger CCCH-type containing 8
Synonyms Zinc finger CCCH domain-containing protein 8, ZC3HDC8, Fliz1, 1G8C6
Observed Molecular Weight36 kDa
GenBank Accession NumberBC032001
Gene Symbol ZC3H8
Gene ID (NCBI) 84524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N5P1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H8, also named as Fliz1, is a novel zinc finger protein preferentially expressed in fetal liver hematopoietic progenitors and in several adult organs, including the thymus. It acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Overexpression of Fliz1 repressed the expression of GATA-3 in vivo and in vitro. GATA-3 might be partly regulated by the intronic regulatory region and Fliz1 in a developmental stage-specific fashion (PMID: 23932780).

实验方案

Product Specific Protocols
WB protocol for ZC3H8 antibody 68282-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols