• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ZCRB1 Polyclonal antibody

ZCRB1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25629-1-AP

Print datasheet

Synonyms

Zinc finger CCHC-type and RNA-binding motif-containing protein 1, SNRNP31

验证数据展示

  • WB analysis using 25629-1-AP

    WB analysis using 25629-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25629-1-AP

    IF Staining of HepG2 using 25629-1-AP

    Immunofluorescent analysis of HepG2 cells using 25629-1-AP (ZCRB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inARPE-19 cells, Neuro-2a cells, Raji cells, SMMC-7721 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25629-1-AP targets ZCRB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ZCRB1 fusion protein Ag22095 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger CCHC-type and RNA binding motif 1
Synonyms Zinc finger CCHC-type and RNA-binding motif-containing protein 1, SNRNP31
Calculated Molecular Weight 217 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC022543
Gene Symbol ZCRB1
Gene ID (NCBI) 85437
RRIDAB_2880169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TBF4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZCRB1 antibody 25629-1-APDownload protocol
IF protocol for ZCRB1 antibody 25629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

A novel protein encoded by ZCRB1-induced circHEATR5B suppresses aerobic glycolysis of GBM through phosphorylation of JMJD5.

Authors - Jian Song
View Article
humanWB

bioRxiv

Role of U11/U12 minor spliceosome gene ZCRB1 in Ciliogenesis and WNT Signaling

Authors - Geralle Powell-Rodgers
View Article