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验证数据展示

  • WB analysis using 67514-1-Ig (same clone as 67514-1-PBS)

    WB analysis using 67514-1-Ig (same clone as 67514-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

  • WB analysis using 67514-1-Ig (same clone as 67514-1-PBS)

    WB analysis using 67514-1-Ig (same clone as 67514-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

  • WB analysis of HT-1080 using 67514-1-Ig (same clone as 67514-1-PBS)

    WB analysis of HT-1080 using 67514-1-Ig (same clone as 67514-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67514-1-Ig (same clone as 67514-1-PBS)

    FC experiment of HepG2 using 67514-1-Ig (same clone as 67514-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ZEB2 Monoclonal antibody (67514-1-Ig, Clone:1D3A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 67514-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ZEB2 Monoclonal antibody, PBS Only

ZEB2 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67514-1-PBS

产品说明书

CloneNo. 1D3A2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

SIP1, Smad-interacting protein 1, SIP 1, KIAA0569, HRIHFB2411

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67514-1-PBS targets ZEB2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ZEB2 fusion protein Ag25477 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger E-box binding homeobox 2
别名 SIP1, Smad-interacting protein 1, SIP 1, KIAA0569, HRIHFB2411
观测分子量 160 kDa
GenBank蛋白编号BC127102
基因名称 ZEB2
Gene ID (NCBI) 9839
RRIDAB_2882735
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60315
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )