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验证数据展示

  • FC experiment of MCF-7 using 83909-5-RR

    FC experiment of MCF-7 using 83909-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZFP161 / ZBTB14 Recombinant antibody (83909-5-RR, Clone:240853G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of A549 using 83909-5-RR

    IF Staining of A549 using 83909-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZFP161 / ZBTB14 antibody (83909-5-RR, Clone: 240853G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83909-5-RR

    Affinity and Kinetic Characterization of 83909-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83909-5-RR against Human ZFP161 / ZBTB14 were performed. The affinity constant is 32.9 pM.

ZFP161 / ZBTB14 Recombinant antibody

ZFP161 / ZBTB14 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83909-5-RR

产品说明书

CloneNo. 240853G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ZFP161 Ready-To-Use IHC Kit. ZFP161 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83909-5-RR targets ZFP161 / ZBTB14 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ZFP161 / ZBTB14 fusion protein Ag16125 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger protein 161 homolog (mouse)
别名 ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14
计算分子量 449 aa, 51 kDa
GenBank蛋白编号BC110519
基因名称 ZFP161
Gene ID (NCBI) 7541
RRIDAB_3671491
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO43829
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.

实验方案

Product Specific Protocols
IF protocol for ZFP161 / ZBTB14 antibody 83909-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols