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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of MCF-7 using 83909-5-RR (same clone as 83909-5-PBS)

    FC experiment of MCF-7 using 83909-5-RR (same clone as 83909-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZFP161 / ZBTB14 Recombinant antibody (83909-5-RR, Clone:240853G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83909-5-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83909-5-RR (same clone as 83909-5-PBS)

    IF Staining of A549 using 83909-5-RR (same clone as 83909-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZFP161 / ZBTB14 antibody (83909-5-RR, Clone: 240853G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83909-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83909-5-RR

    Affinity and Kinetic Characterization of 83909-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83909-5-RR against Human ZFP161 / ZBTB14 were performed. The affinity constant is 32.9 pM.

ZFP161 / ZBTB14 Recombinant antibody, PBS Only

ZFP161 / ZBTB14 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83909-5-PBS

Print datasheet

CloneNo. 240853G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ZFP161 Ready-To-Use IHC Kit. ZFP161 primary antibody included.

产品信息

83909-5-PBS targets ZFP161 / ZBTB14 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ZFP161 / ZBTB14 fusion protein Ag16125 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name zinc finger protein 161 homolog (mouse)
Synonyms ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14
Calculated Molecular Weight 449 aa, 51 kDa
GenBank Accession NumberBC110519
Gene Symbol ZFP161
Gene ID (NCBI) 7541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43829
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.