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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ZFP36 Monoclonal antibody

ZFP36 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5E6

Cat no : CL488-66938

Print datasheet

Synonyms

验证数据展示

  • WB analysis using CL488-66938

    WB analysis using CL488-66938

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66938 (ZFP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNCI-H1299 cells
Planning an IHC experiment? We recommend our IHCeasy ZFP36 Ready-To-Use IHC Kit. ZFP36 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66938-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66938 targets ZFP36 in WB applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, pig
Immunogen ZFP36 fusion protein Ag28473 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name zinc finger protein 36, C3H type, homolog (mouse)
Synonyms
Calculated Molecular Weight 326 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC009693
Gene Symbol ZFP36
Gene ID (NCBI) 7538
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26651
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 ZFP36 antibody CL488-66938Download protocol
Standard Protocols
Click here to view our Standard Protocols