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验证数据展示

  • WB analysis using 68038-1-Ig

    WB analysis using 68038-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HSC-T6 using 68038-1-Ig

    WB analysis of HSC-T6 using 68038-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 68038-1-Ig

    WB analysis of PC-12 using 68038-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68038-1-Ig

    IF Staining of HepG2 using 68038-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFP36L1 antibody (68038-1-Ig, Clone: 1H6G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ZFP36L1 Monoclonal antibody

ZFP36L1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68038-1-Ig

Print datasheet

CloneNo. 1H6G6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat

Applications

WB, IF/ICC, ELISA

Synonyms

Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, HSC-T6 cells, PC-12 cells, U2OS cells, A549 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68038-1-Ig targets ZFP36L1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Rat
Immunogen ZFP36L1 fusion protein Ag30469 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name zinc finger protein 36, C3H type-like 1
Synonyms Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1
Calculated Molecular Weight 338 aa, 36 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberBC018340
Gene Symbol ZFP36L1
Gene ID (NCBI) 677
RRIDAB_2918780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

As a member of the Tristetraprolin (TTP) RNA binding protein family, ZFP36L1 regulate gene expression post-transcriptionally by promoting mRNA decay. In mammals, ZFP36L1 and its family members have been shown to regulate development, cell diferentiation, cell cycle, apoptosis and anti-cancer capability by targeting an extensive overlapping repertoire of mRNAs.

实验方案

Product Specific Protocols
WB protocol for ZFP36L1 antibody 68038-1-IgDownload protocol
IF protocol for ZFP36L1 antibody 68038-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols