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验证数据展示

  • WB analysis using 68038-1-Ig

    WB analysis using 68038-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HSC-T6 using 68038-1-Ig

    WB analysis of HSC-T6 using 68038-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 68038-1-Ig

    WB analysis of PC-12 using 68038-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68038-1-Ig

    IF Staining of HepG2 using 68038-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFP36L1 antibody (68038-1-Ig, Clone: 1H6G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ZFP36L1 Monoclonal antibody

ZFP36L1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68038-1-Ig

产品说明书

CloneNo. 1H6G6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Rat

应用

WB, IF/ICC, ELISA

别名

Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, HSC-T6 cells, PC-12 cells, U2OS cells, A549 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68038-1-Ig targets ZFP36L1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Rat
免疫原 ZFP36L1 fusion protein Ag30469 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger protein 36, C3H type-like 1
别名 Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1
计算分子量 338 aa, 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC018340
基因名称 ZFP36L1
Gene ID (NCBI) 677
RRIDAB_2918780
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07352
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

As a member of the Tristetraprolin (TTP) RNA binding protein family, ZFP36L1 regulate gene expression post-transcriptionally by promoting mRNA decay. In mammals, ZFP36L1 and its family members have been shown to regulate development, cell diferentiation, cell cycle, apoptosis and anti-cancer capability by targeting an extensive overlapping repertoire of mRNAs.

实验方案

Product Specific Protocols
WB protocol for ZFP36L1 antibody 68038-1-IgDownload protocol
IF protocol for ZFP36L1 antibody 68038-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols