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验证数据展示

  • IF Staining of HepG2 using CL488-68038

    IF Staining of HepG2 using CL488-68038

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ZFP36L1 antibody (CL488-68038, Clone: 1H6G6 ) at dilution of 1:600, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-68038

    FC experiment of HeLa using CL488-68038

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ZFP36L1 (CL488-68038, Clone:1H6G6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated ZFP36L1 Monoclonal antibody

ZFP36L1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68038

产品说明书

CloneNo. 1H6G6

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

IF/ICC, FC (Intra)

别名

ZFP36L1/2, ZFP36 L1, TPA-induced sequence 11b, mRNA decay activator protein ZFP36L1, ERF-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68038-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68038 targets ZFP36L1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat
免疫原 ZFP36L1 fusion protein Ag30469 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger protein 36, C3H type-like 1
别名 ZFP36L1/2, ZFP36 L1, TPA-induced sequence 11b, mRNA decay activator protein ZFP36L1, ERF-1
计算分子量 338 aa, 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC018340
基因名称 ZFP36L1
Gene ID (NCBI) 677
RRIDAB_3084392
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07352
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

As a member of the Tristetraprolin (TTP) RNA binding protein family, ZFP36L1 regulate gene expression post-transcriptionally by promoting mRNA decay. In mammals, ZFP36L1 and its family members have been shown to regulate development, cell diferentiation, cell cycle, apoptosis and anti-cancer capability by targeting an extensive overlapping repertoire of mRNAs.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ZFP36L1 antibody CL488-68038Download protocol
FC protocol for CL Plus 488 ZFP36L1 antibody CL488-68038Download protocol
Standard Protocols
Click here to view our Standard Protocols