验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, human placenta tissue, mouse kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human heart tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13118-2-AP targets ZFYVE16 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, zebrafish |
| 免疫原 | ZFYVE16 fusion protein Ag3811 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, FYVE domain containing 16 |
| 别名 | Zinc finger FYVE domain-containing protein 16, KIAA0305, Endosome-associated FYVE domain protein, Endofin |
| 计算分子量 | 1539 aa, 169 kDa |
| 观测分子量 | 230-250 kDa |
| GenBank蛋白编号 | BC032227 |
| 基因名称 | ZFYVE16 |
| Gene ID (NCBI) | 9765 |
| RRID | AB_2288795 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z3T8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZFYVE16 antibody 13118-2-AP | Download protocol |
| IHC protocol for ZFYVE16 antibody 13118-2-AP | Download protocol |
| IF protocol for ZFYVE16 antibody 13118-2-AP | Download protocol |
| IP protocol for ZFYVE16 antibody 13118-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet The Lowe Syndrome Protein OCRL1 Is Required for Endocytosis in the Zebrafish Pronephric Tubule. | ||
Structure Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.
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iScience Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.
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