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验证数据展示

  • IF Staining of HeLa using CL488-13118

    IF Staining of HeLa using CL488-13118

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 ZFYVE16 antibody (CL488-13118) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ZFYVE16 Polyclonal antibody

ZFYVE16 Polyclonal Antibody for IF/ICC
Cat No. CL488-13118

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

DKFZp686E13162, ENDOFIN, KIAA0305, ZFYVE16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13118 targets ZFYVE16 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 ZFYVE16 fusion protein Ag3811 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, FYVE domain containing 16
别名 DKFZp686E13162, ENDOFIN, KIAA0305, ZFYVE16
计算分子量 1539 aa, 169 kDa
观测分子量230-250 kDa
GenBank蛋白编号BC032227
基因名称 ZFYVE16
Gene ID (NCBI) 9765
RRIDAB_3672558
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z3T8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ZFYVE16 antibody CL488-13118Download protocol
Standard Protocols
Click here to view our Standard Protocols