验证数据展示
经过测试的应用
| Positive WB detected in | ROS1728 cells, Y79 cells, PC-12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25004-1-AP targets ZIC1 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | ZIC1 fusion protein Ag13400 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Zic family member 1 (odd-paired homolog, Drosophila) |
| 别名 | ZIC, ZIC1, Zinc finger protein ZIC 1, ZNF201 |
| 计算分子量 | 447 aa, 48 kDa |
| 观测分子量 | ~50 kDa |
| GenBank蛋白编号 | BC104848 |
| 基因名称 | ZIC1 |
| Gene ID (NCBI) | 7545 |
| RRID | AB_3085766 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15915 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZIC1 is a member of the Zinc finger of the cerebellum (ZIC) protein family. In vertebrates, the ZIC gene family plays an important role in neural crest and subsequent cerebellar development. Mutations in ZIC family members have demonstrated that they are essential for proper development of the neural tube, left-right symmetry, and muscle and skeletal development. It has been reported that ZIC1 participates in the regulation of cell proliferation in the dorsal neural tube by inhibition of neural differentiation. Furthermore, ZIC1 is potentially involved in the oncogenesis of undifferentiated nervous system tumors. (PMID:17936758)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZIC1 antibody 25004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
