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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 25004-1-AP

    WB analysis using 25004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25004-1-AP (ZIC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ZIC1 Polyclonal antibody

ZIC1 Polyclonal Antibody for WB, ELISA
Cat No. 25004-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

ZIC, ZIC1, Zinc finger protein ZIC 1, ZNF201

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inROS1728 cells, Y79 cells, PC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25004-1-AP targets ZIC1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen ZIC1 fusion protein Ag13400 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Zic family member 1 (odd-paired homolog, Drosophila)
Synonyms ZIC, ZIC1, Zinc finger protein ZIC 1, ZNF201
Calculated Molecular Weight 447 aa, 48 kDa
Observed Molecular Weight~50 kDa
GenBank Accession NumberBC104848
Gene Symbol ZIC1
Gene ID (NCBI) 7545
RRIDAB_3085766
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15915
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZIC1 is a member of the Zinc finger of the cerebellum (ZIC) protein family. In vertebrates, the ZIC gene family plays an important role in neural crest and subsequent cerebellar development. Mutations in ZIC family members have demonstrated that they are essential for proper development of the neural tube, left-right symmetry, and muscle and skeletal development. It has been reported that ZIC1 participates in the regulation of cell proliferation in the dorsal neural tube by inhibition of neural differentiation. Furthermore, ZIC1 is potentially involved in the oncogenesis of undifferentiated nervous system tumors. (PMID:17936758)

实验方案

Product Specific Protocols
WB protocol for ZIC1 antibody 25004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols