验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, PC-3 cells, SKOV-3 cells |
Planning an IHC experiment? We recommend our IHCeasy ZMPSTE24 Ready-To-Use IHC Kit. ZMPSTE24 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84547-1-RR targets ZMPSTE24 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ZMPSTE24 fusion protein Ag33613 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | zinc metallopeptidase (STE24 homolog, S. cerevisiae) |
| 别名 | FACE-1, FACE1, FACE 1, EC:3.4.24.84, CAAX prenyl protease 1 homolog |
| 计算分子量 | 475 aa, 55 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC037283 |
| 基因名称 | ZMPSTE24 |
| Gene ID (NCBI) | 10269 |
| RRID | AB_3672053 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O75844 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZMPSTE24(CAAX prenyl protease 1 homolog) gene encodes a zinc metalloproteinase involved in the processing of farnesylated proteins(PubMed: 10373325). The significance of ZMPSTE24 in human disease stems from its role as an enzyme necessary for the correct processing and maturation of lamin A ,an intermediate filament component of the nuclear envelope (PubMed: 16297189).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZMPSTE24 antibody 84547-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |