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验证数据展示

  • WB analysis using 84276-1-RR (same clone as 84276-1-PBS)

    WB analysis using 84276-1-RR (same clone as 84276-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84276-1-RR (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84276-1-RR (same clone as 84276-1-PBS)

    IF Staining of HepG2 using 84276-1-RR (same clone as 84276-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84276-1-RR (same clone as 84276-1-PBS)

    IF Staining of HepG2 using 84276-1-RR (same clone as 84276-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84276-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01357-1

    Sandwich ELISA standard curve of MP01357-1

    Sandwich ELISA standard curve of MP01357-1, Human ZNF217 Recombinant Matched Antibody Pair - PBS only. 84276-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag19453. 84276-2-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL

  • Sample test of MP01357-1

    Sample test of MP01357-1

    The mean ZNF217 concentration was determined to be 885 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84276-1-RR

    Affinity and Kinetic Characterization of 84276-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84276-1-RR against Human ZNF217 were performed. The affinity constant is below 1 pM.

ZNF217 Recombinant antibody, PBS Only (Capture)

ZNF217 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84276-1-PBS

产品说明书

CloneNo. 241217E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

别名

ZFP217, Zinc finger protein 217, ZABC1, 241217E3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84276-1-PBS targets ZNF217 as part of a matched antibody pair:

MP01357-1: 84276-1-PBS capture and 84276-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 ZNF217 fusion protein Ag19453 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger protein 217
别名 ZFP217, Zinc finger protein 217, ZABC1, 241217E3
计算分子量 1048 aa, 115 kDa
观测分子量~ 130 kDa
GenBank蛋白编号BC113427
基因名称 ZNF217
Gene ID (NCBI) 7764
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO75362
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis. PMID: 38259608