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验证数据展示

  • WB analysis using 84359-3-RR

    WB analysis using 84359-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84359-3-RR (ZNF638 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84359-3-RR

    IF Staining of HepG2 using 84359-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF638 antibody (84359-3-RR, Clone: 241636C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84359-3-RR

    Affinity and Kinetic Characterization of 84359-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84359-3-RR against Human ZNF638 were performed. The affinity constant is below 1 pM.

ZNF638 Recombinant antibody

ZNF638 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84359-3-RR

产品说明书

CloneNo. 241636C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

zinc finger protein 638, ZFML, Nuclear protein 220, NP220, CTCL associated antigen se33 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells, HepG2 cells, HeLa cells, A431 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ZNF638 Ready-To-Use IHC Kit. ZNF638 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84359-3-RR targets ZNF638 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZNF638 fusion protein Ag35296 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger protein 638
别名 zinc finger protein 638, ZFML, Nuclear protein 220, NP220, CTCL associated antigen se33 1
计算分子量221kDa
观测分子量 270-300 kDa
GenBank蛋白编号NM_014497.4
基因名称 ZNF638
Gene ID (NCBI) 27332
RRIDAB_3671896
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ14966
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404).

实验方案

Product Specific Protocols
WB protocol for ZNF638 antibody 84359-3-RRDownload protocol
IF protocol for ZNF638 antibody 84359-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols